Single-cell alternative splicing analysis with Expendition reveals splicing dynamics during neuron differentiation. Song et al
smRNA-FISH demonstrate presence of constitutive exons or alternative splicing exons in two different cell-types: iPSCs and MNs. For PKM, exon 10 is primary included in iPSCs, but not observed in MNs, whereas exon 9 is primarily observed in MNs but not in iPSCs. For MAP4K4, alternative exon 16 is less included in iPSCs, but more included in MNs.