Data for: Sleeve Gastrectomy Ameliorates Endothelial Function and Prevents Lung Cancer by Normalizing Endothelin-1 Axis and Reduces DNA damage in Obese and Diabetic Rats

Published: 14-02-2020| Version 3 | DOI: 10.17632/vj5469pmv6.3
Rishat Ruzi,
Yacheng Xiong,
Jianwen Li,
Mingwei Zhong,
Qian Xu,
Zhibo Yan,
Jiankang Zhu,
Yugang Cheng,
Sanyuan Hu,
Guangyong Zhang


Background Bariatric surgeries are proved to be effective in preventing and lowering the risk of various types of cancer, yet its underlying mechanism is not clearly elucidated. Methods Sleeve gastrectomy(SG) was performed on obese and diabetic Wistar rats and the postoperative transcriptional and translational alterations of ET-1 axis in their lungs were compared to sham(SH)-operated obese and diabetic rats and age-matched healthy controls(C) to assess the improvements in endothelial function and risk of developing lung cancer at postoperative 4th, 8th and 12th week. Combined with this, the risk was also evaluated using nuclear phosphorylation of histone H2AX(γ-H2AX) focus assay as a marker of DNA damage(double-strand break). Result SG brought a significant reduction to body weight, food intake and fasting blood glucose(FBG), values of oral glucose tolerance test(OGTT) and insulin resistance(IR). Beyond that, ameliorated level of genes and proteins expression of ET-1 axis as well as the reduced DNA damage showed an improved endothelial function and lowered risk of developing lung cancer after the surgery. Conclusion Apart from eliminating metabolic disorders, SG improves endothelial function and plays a protective role in preventing lung cancer via normalized ET-1 axis and reduced DNA damage.


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