supplementary materials: Gentamicin sponge for anti-infection, a controversial old topic and preliminary exploration of the solution, in vitro and in vivo experiments

Published: 03-12-2020| Version 1 | DOI: 10.17632/vpj7vjkz86.1
Contributor:
Junlin Zhou

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supplementary materials: Gentamicin sponge for anti-infection, a controversial old topic and preliminary exploration of the solution, in vitro and in vivo experiments. abstract: Although gentamicin sponge is approved for preventing or treating surgical infection in many countries, the effectiveness of it has still been controversial. Our study was to construct drug-loading and drug-release quantitative equation of gentamicin sponge, in addition, obtain the wound infection prevented and treated scheme of gentamicin sponge. Firstly, sponge was cut into 1×1×0.5cm size and immersed into 40mg/ml, 16mg/ml, 8mg/ml, 4mg/ml, 1.6mg/ml, 0.8mg/ml or 0mg/ml gentamicin solution to evaluate gentamicin-loading of sponge. Secondly, different air-dried gentamicin-saturated sponge was immersed into 10ml 0.9% physiological saline to analyze the drug-release of gentamicin sponge. Thirdly, methicillin sensitive Staphylococcus aureus (MSSA) and Pseudomonas aeruginosa (P. aeruginosa) were used to explore the infection prevented scheme of gentamicin sponge. Finally, femur fractured with wound infection rat model was used to discuss the infection treated scheme. Then, the equation of gentamicin-loading of sponge was: z=(0.03718±0.01672)x+(-4.578e-4±0.06253)y+(-2.50935e-4±1.47521e-4)x2+(0.00303±0.00149)y2+(0.00408±3.52827e-4)xy (R2 was 0.97) and drug-release equation was z=(4.37205±1.18048)x+(-7.05921±3.09628)y+(-0.04596±0.01287)x2+(0.3309±0.07912)y2+(0.31559±0.02754)xy (R2 was 0.95). 1.6 mg/ml air-dried sponge and 0.8mg/ml air-dried sponge were enough to prevent wound infection. Besides, if wound were confirmed to be sensitive bacteria infected, we recommended to use 40mg/ml air-dried sponge, 16mg/ml air-dried sponge or 8mg/ml air-dried sponge to treat.

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