Databases new for "Variations of chloride ion channel related genes during diapause termination in silkworm, Bombyx mori"
Description
Abstract: Instant soaking and refrigerated soaking are commonly used in cocoon production to prevent or break diapause and provide planned silkworm eggs, but their mechanisms have not been fully understood. This study aimed to evaluate the mechanisms by which hydrochloric acid (HCl) promotes embryonic development in silkworm Bomyx mori (B. mori), focusing on the chloride ion (Cl-) and its related gene expression profiles. Our results revealed that the HCl treatment of up to 6 min enhanced hatchability in freshly picked and cold-stored eggs, whereas a slight decrease in hatchability was observed in treated with dimethyl sulfoxide (DMSO) for 40 minutes. Using bioinformatics, molecular biology and genetic engineering techniques, the genes encoding chloride ion channel proteins were cloned and transcriptionally analyzed by quantitative reverse transcription PCR in B. mori 932 strain. The phylogenetic analysis of BmCLIC, BmGluCl, and Bmγ-Cl showed that these genes mainly clustered with Lepidopteran insect homologs, but showed a relatively low conservation from B. mori to mammals. We further explored the mRNA levels of BmCLIC, BmGluCl, and Bmγ-Cl in different egg processing conditions and embryonic developmental stages following HCl and DMSO treatments. Our results revealed that the mRNA expression profiles of BmCLIC, BmGluCl, and Bmγ-Cl increased in 20 h postpartum eggs and refrigerated eggs. Moreover, the expression patterns of BmCLIC, BmGluCl, and Bmγ-Cl were gradually induced from day 4 to day 9 of the embryonic stages after both HCl and DMSO treatments of instant pickling compared to the control. However, HCl treatment did not induce transcriptional activation of these genes in cold-stored eggs, even reducing expression at later embryonic developmental stages. Generally, in the production practice of soaking silkworm eggs with HCl in terms of its chemical composition, except H+, Cl- also plays an auxiliary and important role to further promoting the release of diapause. Key words: Bombyx mori; diapause; hatchability; chloride channel; expression analysis