Long-term survival after cryopreservation of the endangered exceptional species Crotalaria avonensis. Philpott et al., 2025.

Description

Data for publication, "Long-term survival after cryopreservation of the endangered exceptional species Crotalaria avonensis". Data represents long- and short-term survival after cryopreservation for the endangered plant species, Crotalaria avonensis. This study presents an assessment of survival across three variations on the encapsulation vitrification method for 3 – 16 years of cryostorage for shoot tips of the federally endangered exceptional plant species, C. avonensis. In addition, the more recently-developed droplet vitrification method was compared to encapsulation vitrification for short-term survival. Overall, over 92% of banked genotypes survived after long-term cryo-storage, and the droplet vitrification method appears to improve survival over the original encapsulation vitrification procedures. In addition, the long-term survival data validate the previously published guidance that a 40% initial survival after liquid nitrogen exposure will lead to a 95% chance of survival after long-term storage, with 39% initial survival in C. avonensis leading to a 95% probability of survival after long-term storage per genotype. Initial survival after short-term liquid nitrogen exposure appears to be an indicator of long-term survival in storage, which can give practitioners some indication of their banking success.

Steps to reproduce

Shoot tips of Crotalaria avonensis were isolated from tissue culture and cryopreserved using the following methods: encapsulation vitrification (EV), encapsulation vitrification - strips (EVstrips), encapsulation vitrification osmoprotection (EVOP), or droplet vitrification (DV). Survival was determined as the percentage of shoot tips from a cryovial that were green and showed some growth by 4 weeks post-recovery. At the time of banking, for each genotype and cryopreservation method, a subset of shoot tips (10, unless otherwise noted) was transferred to recovery medium after PVS2 exposure and rinsing, and another subset after 1 hour LN exposure, rewarming, and rinsing, to serve as controls. Initial survival for these controls was determined after 4 weeks.

Institutions

• Cincinnati Zoo and Botanical Garden

  OH, Cincinnati

Categories

Funders

• Institute of Museum and Library Services

  District of Columbia, United States

  Grant ID: LG-25-12-0595-12

Licence