Figure 1D - tRNA acylation levels of exponentially growing MG1655 and MG1655relA::HTF measured by Northern blotting analysis
Published: 4 April 2018| Version 1 | DOI: 10.17632/vv3pvnb75h.1
Contributor:
Kenn GerdesDescription
tRNA acylation levels of exponentially growing MG1655 and MG1655relA::HTF measured by northern blotting analysis. Total tRNA was extracted from cells growing in MOPS minimal medium with 0.2% glucose as the carbon source before and after isoleucine starvation. A fraction of the purified tRNA was deacylated (by 100 mM Tris-HCL [pH 9]) and charged and uncharged samples were separated by urea PAGE and transferred to a membrane. tRNAileTUV, tRNAasnUTVW and tRNAtrpT were detected by hybridization with radiolabeled tRNA-specific DNA oligonucleotide probes.
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Southern Blot Analysis