Zophobas morio - abf files- raw UHPLC data - hemolymph and feces

Description

This study explores the metabolic adaptability of Zophobas morio larvae when exposed to a diet containing Styrofoam, a polystyrene-based plastic. Our hypothesis is that the larvae possess metabolic, enzymatic, and genetic tools to degrade Styrofoam; however, this adaptation may result in metabolic imbalances or the accumulation of recalcitrant by-products. By examining metabolic profiles from their hemolymph and feces, we aim to identify biochemical pathways involved in plastic degradation and assess potential risks of metabolic bottlenecks or harmful by-products. Larvae were divided into three feeding groups: WB (wheat bran - 6) – a control group fed a standard wheat bran diet; WBPS (wheat bran and Styrofoam - 5) – a mixed diet group to assess partial substitution effects; PS (Styrofoam only -1) – a group fed solely on Styrofoam to evaluate full adaptation to plastic consumption. Samples were collected from the hemolymph and feces of each group after a defined feeding period. Metabolite extraction was performed using a methanol-based protocol to ensure high recovery and compatibility with UHPLC-MS. Ultrahigh-performance liquid chromatography-mass spectrometry (UHPLC-MS) was employed for non-targeted metabolomic analysis.Data Description and Interpretation:The raw UHPLC-MS data include retention times, mass-to-charge ratios (m/z), and ion intensities for metabolites detected in hemolymph and feces from each feeding group. This allows for detailed comparisons of metabolic profiles across different diets. Utility of the Data: This dataset provides a foundation for understanding the metabolic pathways involved in plastic degradation in Zophobas morio. It is a valuable resource for identifying enzymes or pathways for biotechnological applications, such as engineered microbial systems for plastic waste management. Researchers can also use the data for comparative studies on other plastic-degrading organisms or assess environmental implications of Styrofoam degradation. How the Data Was Gathered: The dataset represents raw UHPLC-MS outputs, including chromatographic retention times, spectral m/z values, and ion intensities. All experiments followed validated protocols for reproducibility, with randomized sample analysis to minimize batch effects and appropriate quality control standards included.

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