Confocal Raman Spectroscopy for determing the severity of SARS-CoV-2 using plasma samples
The following data contains Confocal Raman Spectra of 64 patient's serum samples infected with SARS-CoV-2
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WITec alpha 300R Confocal Raman microspectroscope (Ulm, Germany) was used in the study. The instrument was calibrated with silica. Raman spectra were recorded from these plasma samples with the following spectral parameters – Laser wavelength 532nm, Laser power 8mW, Grating 600 grooves/mm, Acquisition time 5sX10, Objective 50X, and in the spectral range 400 to 4000 cm-1. 8μL of plasma sample was spotted on a CaF2 window and used for spectral re-cording. For every plasma sample, 10-12 spectra were recorded from 10-12 random points from the plasma spot, considering the heterogeneous nature of plasma as a sample. Each of the spectra was interpolated in 600-1800 cm-1 region, corrected for cosmic signals and background noise, base-line corrected and area normalized. The spectra were subjected to multivariate analysis - Principal component analysis (PCA) (an unsupervised tool) and Principal component based linear discriminant analysis (PC-LDA) (supervised tool). The 10 pre-processed spectra from each plasma sample were averaged to obtain a spectrum per plasma sample. Average sera spectra were subjected to Multivariate analysis using Commercial Unscrambler® X software.