Data for: The spatio-temporal organization of mitochondrial F1FO ATP synthase in cristae depends on its activity mode

Published: 31-03-2020| Version 1 | DOI: 10.17632/vxdc37t2cc.1
Contributor:
Karin Busch

Description

Movie S1. Fluorescence recording of single molecule ATP synthase in mitochondria in stable transfected cells labeled at subunit gamma::HaloTagTM with TMRHTL. Images were taken with a frame rate of 59 Hz. Number of images: 3000. Total recording time: 51 s. Condition: medium with 5.6 mM glucose, no inhibition. Movie S2. Fluorescence recording of single molecule ATP synthase in mitochondria in stable transfected cells labeled at subunit gamma::HaloTagTM with TMRHTL. Images were taken with a frame rate of 33 Hz. Number of images: 1605. Total recording time: 48 s. Condition: medium with 5.6 mM glucose at 0.5 h after addition of 2-DG (30 mM). Movie S3. Fluorescence recording of single molecule ATP synthase in mitochondria in stable transfected cells labeled at subunit gamma::HaloTagTM with TMRHTL. Images were taken with a frame rate of 33 Hz. Number of images: 1868. Total recording time: 56 s. Condition: medium with 5.6 mM glucose at 2.5 h after addition of 2-DG (30 mM).

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