Dataset of De Novo Hybrid Berry Transcriptome Profiling and Characterization of Piper sps. (Piper nigrum and Piper longum) using Illumina and Nanopore Sequencing

Published: 21 June 2021| Version 1 | DOI: 10.17632/vyr4r7mxj8.1
Contributors:
Sheeja T E, Shelvy S, Muhammad Fayad, Johnson George, Santhosh J Eapen,
, Dinesh Kumar, Anil Rai

Description

De-novo hybrid Transcriptome analysis using Illumina and Nanopore platform. ◦ Hybrid transcriptome assembly. ◦ Isoform detection and Gene prediction

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Steps to reproduce

The short read assembly is performed with Illumina data using velvet and oases, and short read assembly along with Nanopore and Illumina reads was used for the De-novo hybrid transcriptome assembly using IDP-denovo. A total of 308369 contigs from P. nigrum and 267715 contigs from P. longum were generated. The hybrid transcriptome assembly, Isoform detection, and gene prediction are also processed in the analysis.

Institutions

Indian Institute of Spices Research

Categories

Natural Sciences, Agricultural Plant, Agricultural Biotechnology

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