Electrochemical immunosensors using electrodeposited gold nanostructures for detecting the S proteins from SARS-CoV and SARS-CoV-2

Published: 1 February 2022| Version 1 | DOI: 10.17632/vzb9sn7cc2.1
Lais Canniatti Brazaca,
Amanda Hikari Imamura,
Nathalia Gomes,
Mariana Bortholazzi Almeida,
Desirée Tamara Scheidt,
Paulo Raymundo-Pereira,
Osvaldo Oliveira,
Bruno Campos Janegitz,
Sergio Antonio Spinola Machado,
Emanuel Carrilho


This data is related to the development of an electrochemical immunosensor for the S protein of SARS-CoV and SARS-CoV-2 viruses. For that, screen-printed carbon electrodes (SPCEs) were modified by the electrodeposition of gold nanoparticles using different conditions. Specific anti-S protein antibodies were immobilized in the surface of the electrode using EDC and NHS, with the presence of the analyte being assessed by electrochemical impedance spectroscopy. Immunsensors were tested using biological samples, showing great potential to be applied in clinical samples. Furthermore, the devices were able to detect S protein in a rapid (35 min), sensitive and low-cost (< US$ 0.03 per device) manner.



Universidade de Sao Paulo, Universidade Federal de Sao Carlos


Analytical Chemistry, Diagnosis, Biosensor