The genotypes of green frogs from water bodies of Western Ukraine

Published: 14-12-2018| Version 1 | DOI: 10.17632/w2w2cjcvjp.1
Contributor:
Strus Vasylyna

Description

The file contains genotype data of 91 individuals of green frogs, collected in water bodies of Western Ukraine. Seven microsatellite loci were used: Rrid059А, Rrid082А, Rrid171, RlCA1b5, Res16, Res22, RlCA19

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DNA was extracted from samples of muscle tissue stored in 70% alcohol and also from buccal swabs according to the N. Pindasie et al. method. For the extraction of DNA, we used universal laboratory sets “Diatom™ DNA Prep” produced by the «Laboratoriya Isogen». For the PCR we used already prepared mixes of reagents specially developed for the PCR-amplification – GenePak® PCR Core produced by «Laboratoriya Isogen». Electrophoretic separation of obtained DNA samples was performed in 6% polyacrylamide gel with the usage of Tris-Borate-EDTA buffer (TBE Buffer). To estimate the length of fragments, a DNA plasmid pBR322 of E. coli prepared by HpaII restriction endonuclease was added to each of the gel plates.