Data for: Irreversible alterations in the hemoglobin structure affect oxygen binding in human packed red blood cells

Published: 26 April 2021| Version 1 | DOI: 10.17632/w322sn882h.1
Ewa Szczesny-Malysiak, Jakub Dybas, Aneta Blat, Katarzyna Bulat, Kamil Kus, Magdalena Kaczmarska, Aleksandra Wajda, Kamilla Malek, Stefan Chlopicki, Katarzyna Maria Marzec


BGA - Blood gas results obtained weekly for pRBCs during 8 weeks of storage for N=10. IR results for donor A, B and C - These data include second derivatives spectra of pRBCs samples. The raw spectra were preprocessed by ATR-correction, second derivatve spectra calculation with Savitzky-Golay smoothing and normalization. Biochemical analysis - File with the data from the biochemical analysis - glucose and lactate concentrations. Each measurement was repeated twice for each sample measured weekly during 8 weeks. Fig 5 UVVis raw spectra: is set of a UVVis absorption spectra which represents rate of reoxygenation of pRBCs stored for 1 and 5 weeks treated with subsequent doses of sodium dithionite. In each group N was equal to 3. Fig 2 UVVis raw spectra: comprises a set of UVVis absorption spectra obtained for pRBCs stored for 2, 4, 6 and 8 weeks. In each group N was equal to 3. Fig 3 RS spectra: represents a set of Raman spectra obtained for pRBCs stored for 2, 4, 6 and 8 weeks with 488 nm excitation wavelength. In each group N was equal to 3, each sample was measured in 10 randomly chosen spots, each spectrum was accumulated 10 times. LC-MS/MS data - Detection of intracellular metabolites was performed as defined in manuscript. Briefly, an aliquot of 50 μL of pRBCs was centrifuged (500xg, 10 min, RT, no braking) and divided into two fractions – pure fraction of RBCs (marked as RBC in excel file) and SAGM solution supernatant (marked as KON in excel file).



Spectroscopy, Metabolomics, Fourier Transform Infrared Spectroscopy, UV-Visible Spectroscopy, Biochemical Analysis, Liquid Chromatography Mass Spectrometry, Resonance Raman Spectroscopy