Dataset for taxonomic and functional profiling of metagenome grown in secondary sludge of pulp-paper mill wastewater treatment system
Description
Pulp-paper mills (PPMs) generate a large amount of secondary biosludge during the treatment of chlorolignin-contaminated wastewater. However, the complex nature of pollutants and the functional potential of microbial communities thriving in the activated sludge of PPMs remain poorly studied. Activated sludge samples were randomly collected from five different locations within the aeration tanks and combined to form a composite sample for further analysis. Physicochemical characterization and GC-MS analysis of the sediment samples revealed the presence of heavy metals and various chloroorganic pollutants. Simultaneously, high-throughput sequencing (HTS) of the V3–V4 hypervariable region of the 16S rRNA genes derived from the collected sludge sample was performed on the Illumina MiSeq platform. The present dataset includes raw sequencing data (FASTQ files), analysis reports, OUT Table, pie charts, rarefaction curve, and a Krona chart. Additionally, it provides functional annotation tables derived from KEGG, EC, and MetaCyc databases using PICRUSt2 analysis. Taxonomic analysis of the metagenomic sequence data revealed that Proteobacteria was the dominant phylum in the sludge. In addition, other phyla, such as Bacteriodetes, Acidobacteria, Planctomycetes, Chlorolfexi, Actinobacteria, and Vercrucomicrobia were also recorded within the range between 13.27% and 4.1% in sludge. At the genus and species levels, the most abundant genus was an unclassified member (3.62%) of the family Rhodospirillaceae. This unclassified communities could be target taxa in future classification. PICRUSt2-based functional annotation identified a variety of genes, enzymes, and metabolic pathways associated with microbial adaptation and survival in stress-prone environments. This research provides valuable insights into the nature of organic and inorganic pollutants, the composition of bacterial communities, and their potential functional roles in chlorolignin waste-contaminated environments. Future research should focus on the isolation and enrichment of the promising microbial species characterized in this study, as they hold significant potential for developing effective in situ bioremediation strategies