Wachendorfia paniculata
Description
These are the raw data files associated with our manuscript "A combination of pollen mosaics on pollinators and floral handedness facilitates the increase of outcross pollen movement." Authors: Corneile Minnaar and Bruce Anderson, Journal: Current Biology. In this manuscript, we track pollen movement in a handed flower (Wachendorfia paniculata) using quantum dots. We found that handedness leads to outcrossing, because left- and right-handed morphs place most of their pollen on different sides of the pollinators. However, the partial separation of stamens and style also results in two dimensional pollen quality mosaics on each side of carpenter bee pollinators, generating hotspots and coldspots of outcrossed pollen. Similar mosaics were not found on honeybee pollinators. Outcrossed pollen receipt was much higher than expected because stigmatic positions are fine-tuned to match the outcross pollen-hotspots on carpenter bees. Exploitation of these pollen mosaics enables plants to increase the probability of between-morph (i.e. disassortative), outcross pollen-movement beyond the expectations of enantiostyly. Here, we have included the raw data files and their metadata files for all analyses and data-driven figures in the manuscript: Figure 2 in manuscript: The top half of this figure shows the positions of pollen grains found on bee visitors to Wachendorfia paniculata and also the origins of each pollen grain found (i.e. which anther from which morph). The data file associated with this figure is pollen placement coordinates data.csv, while the metadata file associated with these data is pollen placement coordinates metadata.txt. The bottom half of this figure shows the relative positions of stigmas and anthers for 20 left and 20 right handed flowers. The data file associated with these data is anther-stigma coordinates - data.csv while the metadata file associated with those data is anther-stigma coordinates - metadata.txt Figure 3 is a heatmap showing pollen densities and where most within- and between- morph pollen is found on bee wings. The data file for this are the same as for the top half of figure 2 (pollen placement coordinates data.csv, while the metadata file associated with these data is pollen placement coordinates metadata.txt) Figure 4 shows the contributions of different anthers from different morphs to stigmatic pollen loads. The data file associated with this figure is Stigmatic pollen data.xlsx while the metadata file associated with these data is Stigmatic pollen metadata.docx. We also calculated rates of geitonogamy which are reported in the main text of the manuscript. The data file associated with this is Self_pollen data.xlsx while the metadata file associated wit these data is Self_pollen_metadata.txt