Exogenous abscisic acid mitigates chilling injury in zucchini during cold storage by eliciting a time-dependent shaping of specialized metabolites
Description
SUPPLEMENTARY MATERIAL File S1. References on the detailed description of the UHPLC/QTOF-HRMS approach applied on zucchini exocarps samples. Table S1. Dataset of annotated compounds by UHPLC/QTOF-HRMS approach, using the database PlantCyc (https://plantcyc.org). For each compound, the metabolite name, abundance values, retention time (min), mass (u), and molecular formula were provided. Table S2. List of VIP markers associated with OPLS models on the untargeted metabolic profile of exocarps from zucchini fruits under cold storage (at 0, 1, 5, and 14 days) untreated (control) or treated with ABA (ABA). For each compound, the VIP score (threshold = 1.0), standard error, and logFC values of ABA vs. control were provided. Table S3. List of significant compounds subjected to pathway analysis. Compounds show a differential abundance (FC > 2 or FC < -2) and statistically significant differences according to one-way ANOVA and Tukey HSD post hoc test (p < 0.05) with respect to harvest conditions at day 0. For each compound, the metabolite name, logFC values of each treatment with respect to day 0, and the ontology were provided. Table S4. Dataset of annotated compounds by UPLC/QTOF-MS approach, using the database Phenol Explorer (https://phenol-explorer.eu). For each compound, the metabolite name, phenolic class and subclass, abundance values, retention time (min), mass (u), and molecular formula were provided. Table S5. List of VIP markers associated with OPLS models on the phenolic profile of exocarps from zucchini fruits under cold storage (at 0, 1, 5, and 14 days) untreated (control) or treated with ABA (ABA). For each compound, the VIP score (threshold = 1.0), standard error, and logFC values of ABA vs. control were provided. Figure S1. Figure S1. Hierarchical cluster analysis of the untargeted phenolic profile of exocarps from zucchini fruits under cold storage (at 0, 1, 5, and 14 days) untreated (control) or treated with ABA (ABA). Clustering was performed according to the fold change-based heatmap, baselined to the median values of all samples (Euclidean distance, Ward’s algorithm).