GDF15-GFRAL orchestrates glutathione synthesis via NRF2-elicited GCLC activation to impede cuproptosis

Published: 25 July 2023| Version 1 | DOI: 10.17632/w7t7wmmgm7.1
Yabing Nan


Untargeted metabolomic data


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The raw MS data were converted to MzXML files using ProteoWizard MSConvert before importing into freely available XCMS software. Compound identification of metabolites was performed by comparing the accurate m/z values (<10 ppm) and MS/MS spectra with an in-house database established with available authentic standards. After sum normalization, the processed data were subjected to multivariate data analyses, including Pareto-scaled PCA and orthogonal partial least squares discriminant analysis (OPLS-DA), with an R package (ropls). The variable importance in projection (VIP) value of each variable in the OPLS-DA model was calculated to indicate its contribution to the classification. Student’s t test was applied to determine the significance of differences between two groups of independent samples. VIP > 1 and P value < 0.05 were used as the criteria to identify significantly changed metabolites. Pearson correlation analysis was performed to determine correlations between two variables.


Chinese Academy of Medical Sciences and Peking Union Medical College