Kinetic basis for DNA target specificity of CRISPR-Cas12a. Strohkendl et al.

Published: 19-07-2018| Version 1 | DOI: 10.17632/w8d5fn5vxn.1
Isabel Strohkendl


This dataset includes the raw data used in Strohkendl et al., 2018, and is organized by figure within the publication. There are two types of data presented: gel images and fluorescence data. The images either show binding and dissociation of Cas12a and its radiolabeled DNA substrate on native polyacrylamide gels or cleavage of a radiolabeled DNA substrate by Cas12a on denaturing polyacrylamide gels. The fluorescence data reports on 2-aminopurine fluorescence increase as a function of R-loop formation. Both types of data are used to determine the rates binding, cleavage, and dissociation of Cas12a while targeting a short DNA duplex.