Data for: Refractive index-assisted UV/Vis spectrophotometry to overcome spectral interference by impurities: applications from impurity analysis to salinity and protein measurements

Published: 2 January 2021| Version 1 | DOI: 10.17632/wbwtp3cx9k.1


It is a study on matrix effects and a new refractometric method that can help analytical techniques like UV/Vis spectrophotometry deal with matrix effects, specifically, spectral interference. Spectral interference due to matrix impurities is a primary reason that hinders the large-scale application of simple analytical techniques like UV/Vis spectrophotometry in complex samples like those in food, beverage, environmental and other industries. There are lengthy studies on the effects of possible interferents in specific cases. However, current approaches to ensure the absence of huge spectral interference errors are either slow or costly unless all possible impurities are known. We have introduced a simple, cost-effective, and rapid, refractive index-based technique which can assist UV/Vis spectrophotometry for detecting and reducing spectral interference in many of these cases. We verified it in more than ten solvents. We also performed more studies on its application in impurity analysis and its capability in detecting interference errors in the standard UV spectrophotometric methods for protein concentration measurement and salinity determination. We hope that the results would encourage the use of this new technique in certain branches of analytical chemistry. This dataset contains the data which was used for the figures and discussion of the results in the original manuscript. It includes, among others, 1) The UV spectra and refractometric measurements of benzene and N, N-Dimethylaniline in cyclohexane 2) Lorentz Lorenz mixing rule applied to refractometric measurements of benzene in ten different solvents (acetonitrile, n-butanol, cyclohexane, CCl4, p-xylene, toluene, o-xylene, chlorobenzene, N, N-Dimethylaniline and aniline) 3) Qualitative analysis of impurity compounds using Pearson's correlation coefficient, refractometry and UV spectrophotometry 4) The refractometric and UV spectrophotometric standard curves of protein and artificial seawater. Interference effects of DNA, RNA, lysozyme and Triton X-100 on protein (BSA) analysis and nitrates and nitrites on salinity measurement are included in the original paper.



Spectroscopy, Protein, Molecular Spectroscopy, Ultraviolet Spectrophotometry, UV-Visible Spectroscopy, Absorption Spectroscopy, Refractive Index, Salinity, Spectrophotometry, Impurity Characterization, Matrix, Pearson Correlation Coefficient