RNA-Seq of Mouse Models - Environmentally-Induced T2D and Control

Published: 31 August 2021| Version 1 | DOI: 10.17632/wc32wwvdwk.1


This is a study that is focusing on Alternative Splicing events occurring in the brain and liver tissues of Western Diet (WD) that got environmentally-induced type 2 diabetes (T2D) and a corresponding control. Total RNA is from the dissected liver and brain samples is isolated with the help of the Qiagen’s RNeasy Mini Kit. TruSeq RNA v2 is used for the RNA-Seq library preparation, including isolating mRNA and preparation for sequencing. RNA integrity number (RIN) on an Agilent 2500 BioAnalyzer is used for RNA quality validation. Samples are deep sequenced on an Illumina HiSeq 2000 using 2 lanes for each sample to achieve close to 100 million 75 bp paired-end reads per sample. The RNA sequencing analysis pipeline consists of Trimmomatic with default settings to remove the low quality reads, Tophat v2 to align on GRCm38.p5, and Cufflinks v2 to quantify and reassemble expression levels. Dataset consists of processed data along with isoform lists of highly-differentiated data (>5-fold)