Dataset for data in brief: Thermoacidophilic Microbial Inoculum Enhanced Composting Efficiency of Lignocellulosic waste.
Description
The role of thermoacidophic effective microbes (tEM) used as microbial innoculants during composting was assesed under two experimental condtions; 1) tEM with shadding (tEMA), 2) tEM without shadding (tEMB) and control group - no tEM with out shadding (C). Treatrment of thermoacidophilic microorganisms during composting of lignocellulosic waste improved microbial diversity and microbial abundance. The improved microbial structure subsequently enhanced composting efficiency (composting rate and quality of the final product). Gas/Biogas production: Hydrogen sulphide (ppm), carbon dioxide (%), oxygen (%), and methane (%) production were measured using a biogas analyzer (Optima7 MRU Instruments Inc. Emission monitoring systems, Humble, Texas, USA). Ammonia gas was analyzed by pumping 50 cm3 biogas through 2% Boric acid (pH of 4.5) using ETG 6900 P Ammonia (Etg Risorse & Tecnologia, Carpignano 23 Montiglio (AT) – Italy). And ammonium borate complex titrated with 0.05N H2SO4 and concentration of ammonia gas was calculated based on moles of standard acid consumed. Biogas evolution has a direct relationship with the microbial decomposition of organic. High concentration of biogases like hydrogen sulphide, methane and ammonia, could indicate anaerobic condition. Aerobic decomposition is a more efficient way of composting since most composting microbes are aerobic. Besides, aerobic composting reduces emission of biogas and thus enhance environmental suitability. Microbial evolution data was obtained over 120d composting period through metagenomic DNA sequencing and culture-based microbial enumeration at thermophilic and mesophilic temperatures. Compost treatment with microbial innoculum (tEMA and tEMB) enhanced microbial diversity and abundance, especially during thermophilic phase compared to control group (C). Compost chemical analysis was performed using inductively coupled plasma, atomic emission spectroscopy (ICP-AES) on Perkin Elmer, Optima 8300 (Thermo Fisher Scientific Solutions Co., Ltd. Gwangpyeong-ro, Gangnam-gu, Seoul, Republic of Korea). Heavy metal concentration was used as an indicator of compost quality. Germination index (Gi) at compost concentrations of 25%, 50%, and 75% and global germination index (GI) (the average Gi at 50% and 75%) were calculated (Gariglio et al., 2002; Oktiawan and Zaman, 2018). The treatment groups showed higher germination index compared to control group. In addition, the treatment groups enhanced the population of beneficial microbes in the final compost while suppressing harmful microbial groups. This data is part of the research article (“Metagenomic Analysis Reveals Enhanced Biodiversity and Composting Efficiency of Lignocellulosic Waste by Thermoacidophilic Effective Microorganism (tEM)” (Ajuna et al.)). The data is important for evaluating composting efficiency and quality of the final compost product.