Model of the Bacillus anthracis S-layer EA1

Description

The Gram-positive and spore-forming bacterium Bacillus anthracis is the etiological agent of anthrax, a deadly disease that today affects mostly wildlife and livestock. Its vegetative cells are covered in a surface layer or S-layer composed of two mutually excluding proteins, Sap and EA1, present in the exponential and stationary growth phases respectively. Using X-ray crystallography we generate a model of the EA1 monomer (PDB ID: 8OPR) that shows the EA1 S-layer assembly domain consists of a beads-on-a-string architecture of 6 immunoglobulin-like domains (D1 - D6), where calcium binding structures interdomain contact loops and allows the protomers to adopt their assembly-competent conformation. Here we deposit the 3D coordinates of the EA1 S-layer model, generated based on a low resolution 3D cryoEM map, and docking of the EA1 X-ray structure and rigid body domain reorganisation of EA1 domain D2 (res 292-396), and validation by directed mutagenesis. See Sogues et al. 2023 "Structure and function of the EA1 surface layer of Bacillus anthracis" for experimental details and full description of biological data.

Steps to reproduce

See Sogues et al. 2023 "Structure and function of the EA1 surface layer of Bacillus anthracis" for experimental details and full description of biological data.

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