biochemical data - Lessonia spicata - Valparaíso, Chile
Description
This dataset includes the ecophysiological and biochemical responses of Lessonia spicata (Ochrophyta) during a natural light deprivation event caused by a solar eclipse. We measured in vivo chlorophyll a (Chla) fluorescence, photoinhibition, and photosynthetic capacity, along with pigment content, phenolic compound accumulation, and antioxidant capacity, to evaluate short-term photosynthetic adjustments. We hypothesized that this abrupt, short-term light deprivation would trigger enhanced ecophysiological adjustments, including elevated production of the compounds of the secondary metabolism as Chla – Chl c – Carotenoids - Poly
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In the intertidal zone of Playa Cochoa, Valparaíso Bay (32°57'19.0"S; 71°32'52.4"W), specimens of Lessonia spicata (Suhr) Santelices (Ochrophyta) were collected at 0.1 to 0.4 m above sea level with a minimum separation of 2 meters between individuals. The samples were collected in the morning (08:00 hours) coinciding with a total solar eclipse, covering approximately 95% of the sampling site. During the experiment period, six thalli of L. spicata (each ~10 g fresh weight) were individually placed in 2.5 L plastic containers filled with filtered seawater. The temperature of the containers was controlled by aeration to simulate natural intertidal conditions. The experiment design consisted of two main phases: (i) Solar Exposure (SE), where all six replicates were exposed to natural solar irradiance between 10:00 and 15:00 local time; and (ii) Recovery Phase (RP), from 15:00 to 16:00, coinciding with the peak and post-peak of the solar eclipse. During the RP, three replicates were subjected to attenuated solar irradiance (<33%) using mesh screens (Mesh treatment – M), while the remaining three replicates were placed under complete solar obscuration conditions (Non-mesh treatment – NM) (Fig. 1). To measure physiological responses and biochemical assays all samples preserved in liquid nitrogen and transported to Laboratory at HUB-Ambiental Playa Ancha University.
Institutions
- Universidad de Playa Ancha