CLAP
Description
Companion datasets for "Denaturing purifications demonstrate that PRC2 and other widely-reported chromatin proteins do not appear to bind directly to RNA in vivo". The following data are organized by folders, in the Figures that they appear. Tables containing various column metrics of CLIP and CLAP experiments (enrichment value, p-value, expression levels, and number of reads) for each detected 100-nucleotide RNA window. Tables labeled "PlusTag" or "MinusTag" indicate experiments in which a Halo-tagged protein was transfected into a human cell line (PlusTag, +tag) mixed with untransfected mouse cells or transfected into mouse cells and mixed with untransfected human cells (MinusTag, -tag); windows were computed over all annotated human RNAs. "Halo-" or "Spy-" prefixes are designated for proteins in which both HaloCLAP and SpyCLAP were performed (i.e., performed on either Halo-tagged or Spy-tagged proteins, respectively.) Unless otherwise noted, all other experiments were performed with HaloCLAP. Full-length gel images of scanned phosphor screens are provided for all CLIP and CLAP-related experiments involving radiolabeling of RNA-protein complexes by 32P (Phosphorus-32).