Screening milling conditions in bottle fermentations for consolidated bioprocessing of sugarcane bagasse
Description
This dataset contains experimental results from a screening study evaluating ball-milling conditions for ex-situ cotreatment of sugarcane bagasse aimed at enhancing carbohydrate solubilization during fermentation. This is a complementary work step to the paper entitled “Enhanced lignocellulose solubilization via consolidated bioprocessing and ex-situ cotreatment at increased solid loadings”. The study investigates the impact of key milling parameters, including milling time, bead size and composition, bead-to-solids ratio, moisture content, and mill type, on biomass comminution and subsequent fractional solubilization of total carbohydrates (FSTC). Experiments were conducted using two ball-milling devices (Retsch MM500 and MTI SFM-3) under different operational configurations. The data enable evaluation of process trade-offs between milling intensity and solubilization efficiency, supporting the identification of low-energy, high-performance pretreatment strategies for lignocellulosic bioprocessing. Here are available a spreadsheet containing all of the data generated along with figures, methods and a results and discussion section for these generated results.
Files
Steps to reproduce
The material and methods section contains detailed data enabling how to reproduce the data. It is necessary to ferment comminuted sugarcane bagasse with cocultures of Clostridium thermocellum and Thermoanaerobacterium thermossacharolyticum. Biomass samples consisted of sugarcane bagasse at varying stages: unfermented, once-fermented, and subjected to different processing schemes, including: F (Fermentation only) – unmilled control MF (Mill–Ferment) – milling prior to fermentation FMF (Ferment–Mill–Ferment) – milling applied between two fermentation steps FF (Ferment–Ferment) – double fermentation without milling Milling performance was initially screened qualitatively based on particle size reduction and homogeneity, followed by quantitative assessment through fermentation assays. Bottle fermentations were conducted in triplicate under controlled conditions, and carbohydrate solubilization was calculated from compositional analysis of solids and liquid fractions. Notes for Reproducibility -Maintain anaerobic and thermophilic conditions during fermentation. -Moisture content strongly affects milling efficiency—control it carefully. -Use triplicates for all fermentation experiments. -Ensure consistent bead material and milling chamber geometry across experiments.
Institutions
- Universidade Estadual de Campinas (UNICAMP)São Paulo, Campinas
- Dartmouth CollegeNew Hampshire, Hanover