Data for: Assessment of metabolic variability and diversity present in leaf, peel and pulp tissue of diploid and triploid Musa spp.

Published: 27-04-2020| Version 1 | DOI: 10.17632/wpgvvnp3dz.1
Contributors:
Paul Fraser,
Harald Schöny,
Delphine Amah,
Allan Brown,
Rony Swennen,
Margit Drapal

Description

The files contain data obtained from GCMS (single quadrupole), UPLC-PDA and LCMS (LC-QTof). The sample preparation with internal standard and sample analysis was performed as previously published for Musa tissue (Drapal et al., 2019). Samples were randomised and analysis performed in batches each including 20 samples, a quality control and an extraction blank. Aliquots for LC-ESI-QqTOF analysis (700 µl aqueous phase) were dried down and resuspended in methanol/water (100 µl, 1:1, v/v) under the addition of an internal standard. Homogentisic acid (5 µg/sample) was used for Fougamou and Mbi egome and genistein (2.5 µg/sample) for the diversity panel. Aliquots for UPLC-DAD analysis (700 µl organic phase) were dried down and resuspended in ethyl acetate/acetonitrile (1:9, v/v, leaf: 100 µl; peel and pulp: 50 µl). Aliquots for of peel and pulp sample extracts (140 µl aqueous phase) were dried down with an internal standard (d4-succinic acid, 10 µg/sample) and derivatised before analysis by GC-MS (EI, single quadrupole) in splitless mode with a temperature gradient of 70−325°C (Price et al., 2016). Data analysis and metabolite identification was also performed as previously reported for Musa tissue (Drapal et al., 2019). The resulting data tables comprised relative quantities (to the relevant internal standard) of metabolites detected by GC-MS and LC-MS and absolute quantities for metabolites measures by UPLC-DAD. All metabolites were expressed relative to the sample weight (µg/g dry wt.).

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