Data set of Acute Oral Toxicity and Subacute Neurotoxicity Pre-clinical Risk Assessments on Sprague Dawley Rats Treated with Single Dose or Repeated Doses of Flavanoid-Enriched Fraction Extracted from Oroxylum Indicum
Description
Here, we present the data set of liquid chromatography-mass spectroscopy (LC-MS), relative organ weight (ROW), hematology, biochemical and histopathological parameters of flavanoid-enriched fraction (FEF)-treated Sprague Dawley (SD) rats. The data set was generated from three study groups: Sighting Study, Acute Toxicity Study and Subacute Neurotoxicity Study with study duration of 14-days (Sighting Study and Acute Toxicity Study) and 28 days (Subacute Neurotoxicity Study) by strictly following the procedures set in Organisation for Economic Co-operation and Development (OECD) Guidelines 420 and Guidelines 424 in vivo. Rats in sighting study were treated with dosage of 5, 50, 300 and 2000 mg/kg FEF (n=1/dosage/gender), respectively, and was observed for mortality, toxicity signs and behavioral changes. For acute toxicity study, the highest dosage from sighting study where the animal survived were selected as the dosage, with higher number of animals (n=5/dosage/gender). Meanwhile, for subacute neurotoxicity study, male and female SD rats (n=5/dosage/gender) were treated with dosage of 50 mg/kg for 28 days. Rat’s behavior were observed daily, body weight were measured weekly, while organs weight were harvested at the end of the study (Day 15). Whole blood were collected at the end of the study via cardiac puncture into EDTA tubes for hematological evaluation that includes red blood cells (RBC), hemoglobin (Hb), packed cell volumes (PCV), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), platelet, white blood cells (WBC) count and WBC differentials. Meanwhile, blood serum were collected in slow sand filter (SST) tubes for biochemical evaluation such as total protein (TP), albumin, bilirubin, alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT). All vital organs (brain, liver, kidneys, heart, lungs and reproductive organs) also were collected at the end of the study for histopathological assessments.