Impact of tissue inhibitor of matrixmetalloprotease-1 (TIMP-1) protein on the proliferation and gene expression of rabbit Achilles tenocytes and rabbit adipose-derived stem cells in vitro

Published: 17 January 2025| Version 1 | DOI: 10.17632/wth9gfzktv.1
Contributor:
Johanna Buschmann

Description

In order to know the effect of TIMP-1 on in vitro cell culture of rabbit Achilles tenocytes and rabbit adipose-derived stem cells, we assessed their proliferation with Alamar Blue® assay and furthermore report the effect on gene expression of Col1A1 (extracellular matrix marker collagen I), MKI67 (proliferation marker ki67), TNMD (tenomodulin) and ALP (alkaline phosphatase, a marker for early osteogenesis), respectively, the latter because TIMP-1 has been reported to affect stem cell fate towards osteogenic commitment. As reference gene, 18S was utilized. These experiments have been performed at different concentrations of TIMP-1 supplementation; i.e. at 1, 10, and 100 ng/mL TIMP-1. The same experiment without TIMP-1 served as control.

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Institutions

Universitat Zurich, UniversitatsSpital Zurich

Categories

Gene, Collagen, Cell Proliferation, Adipose-Derived Mesenchymal Stem Cell, Achilles Tendon

Funding

Swiss National Science Foundation

310030_197578

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