Data Figure 3_BLI_Monoclonal antibodies binding data for SARS-CoV-2 proteins

Published: 19 April 2022| Version 1 | DOI: 10.17632/wwjv6xpzx6.1
Contributors:
Nawneet Mishra,
Joan Teyra

Description

Processed data for Figure 3. Data are in excel format which includes data points and fitting points for different antibodies used. The binding responses were analyzed by subtracting data from reference, and data were globally fitted with a 1:1 binding model using ForteBio’s Data Analysis software 9.0

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Binding Kinetics (BLI) BLI experiments were performed on an Octet HTX instrument (Sartorius) at 1000 rpm and 25 °C, were 2-20 microgram per milliliter of SARS-CoV2 antigens were covalently captured on AR2G biosensors (Sartorius, 18-5092) by amine-reactive coupling chemistry, and unbound reactive N-hydroxysulfosuccinimide (NHS) esters on the biosensors were quenched for 600 s by 1M ethanolamine (pH 8.5)[3, 4]. The biosensor probe were equilibrating with assay buffer (PBS, 1% BSA, 0.05% Tween 20), dipped for 600 s into wells containing serial 3-fold dilutions of IgGs, and were transferred back into assay buffer for 600 s of dissociation. The binding responses were analyzed by subtracting data from reference, and data were globally fitted with 1:1 binding model using ForteBio’s Data Analysis software 9.0.

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Biochemical Assay

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