TNF synergy with IL-17A & IL-17F stimulation of MSCs

Published: 24 March 2025| Version 1 | DOI: 10.17632/wws2cx74zw.1
Contributor:
Nicole McDermott

Description

The IL-17 pathway is implicated in axial SpA and targeted by multiple biologics in the clinic, we wanted to assess the downstream effects of IL-17A and IL-17F on entheseal stroma as these cytokines have been identified to drive inflammation and possibly new bone formation. Specifically we wanted to investigate the synergistic effect of IL-17A and IL-17F in combination with TNF. Bulk-seq was performed on spinal MSCs isolated from human spinal entheseal bone and soft tissue, MSCs were stimulated with IL-17A, IL-17F and TNF alone and in combination to assess the synergistic effects of these cytokines on stromal function and activation.

Files

Institutions

Categories

Mesenchymal Stem Cell, Tumor Necrosis Factor, Spondyloarthropathy, IL-17A Signaling Pathway

Funders

Licence