End product formation of glucose in the presence of organic acids by Thermoanaerobacter pseudethanolicus

Description

Degradation of glucose in the presence of organic acids by Thermoanaerobacter pseudethanolicus. Data shows the effect of various environmental factors on the conversion of organic acids to their corresponding alcohols.

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The dataset was obtained by investigating thermophilic bacteria, Thermoanaerobacter pseudethanolicus. While working with thermophilic anaerobes in general, with focus on the effects of various fatty acids on growth, our data indicated that some of bacteria within the genera of Thermoanaerobacter and Caldanaerobacter could use the fatty acids as electron acceptor, resulting in formation of their corresponding alcohol. In this study, we cultivated Thermoanerobacter pseudethanolicus on glucose (65°C, pH 6.5) in the presence of C2 - C6 fatty acids under anaerobic conditions (using the Hungate techique). Without adding any fatty acid to the culture the strain produced a mixture of ethanol and acetate (together with hydrogen and carbon dioxide). When the strain was cultivated on glucose and various fatty acids the strain produced significantly less amount of ethanol but added the produced reducing power on the volatile fatty acid present in the medium. To further confirm that fatty acids were indeed converted to their corresponding alcohols, the volumetric activities of oxidative enzyme reactions using NAD+ and NADP+ as a cofactor when T. pseudethanolicus is cultivated on either glucose (20 mM) or glucose supplemented with selected carboxylic acids were performed. Finally, a detailed 13C NMR spectra of T. pseudethanolicus cultivated on glucose (20 mM) supplemented with 13C1-labled butyrate over a period of 72 hours were performed.

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