Raw Western Blot and PCR Gel
Description
Raw Western Blot and PCR Gel related to manuscript "Circular RNA circPLOD2 regulates pericyte function by targeting the transcription factor KLF4"; Simone Franziska Glaser 1,2,3, Andre Brezski 4,†, Nina Baumgarten 1,2,3,†, Marius Klangwart 1, Andreas W. Heumüller 1, Ranjan Kumar Maji 1,2,3, Matthias S. Leisegang 2,5, Stefan Guenther 2,3,6 Christoph M. Zehendner 1, David John 1,2,3, Marcel H. Schulz 1,2,3,#, Kathi Zarnack 4,#, Stefanie Dimmeler 1,2,3,#,* 1 Institute of Cardiovascular Regeneration, Center of Molecular Medicine, Goethe University, Frankfurt, 60590 Germany 2 German Center for Cardiovascular Research DZHK, partner site Frankfurt Rhine-Main Germany, 3 Cardiopulmonary Institute, Goethe University Frankfurt, 60590 Germany 4 Buchmann Institute for Molecular Life Sciences (BMLS) & Institute of Molecular Biosciences, Goethe University Frankfurt, 60438 Germany 5 Institute for Cardiovascular Physiology, Goethe University Frankfurt, 60590 Germany 6 Bioinformatics and Deep Sequencing Platform, Max Planck Institute for Heart and Lung Research, Bad Nauheim, 61231 Germany †, # contributed equally * Lead contact Original western blots of HIF1α (#14179; Cell Signaling; 1:1000) and GAPDH (#2118S; Cell Signaling; 1:1000). Proteins were isolated from human brain microvascular pericytes incubated under normoxic (21% O2, 5% CO2, 24 h) or hypoxic (1% O2, 5% CO2, 24 h) conditions. PageRuler Plus Prestained Protein Ladder (#26619; Thermo Scientific) was used; n=2 Orignal gel showing PCR products on total RNA from human brain microvascular pericytes with RNAse R (RNase R +) or without (RNase R -). circPLOD2 was amplified with specific primers across the back-splice junction. Linear PDGFR-β and VEGFA mRNAs were used as controls.