Data for: Comparisons of the pharmacokinetic and tissue distribution profilesof withanolide B after intragastric administration of the effective part of Datura metel L. in normal and psoriasis guinea pigs

Published: 30-08-2018| Version 1 | DOI: 10.17632/x3vkrx9hcm.1
Contributors:
Haixue Kuang,
Lijie Zhang,
Lianrong Yang,
Xin Meng,
Bingyou Yang

Description

A simple, highly sensitive ultra-performance liquid chromatography- electrospray ionization-mass spectrometry (LC-ESI-MS) method has been developed to quantify of withanolide B and obakunone (IS) in guinea pig plasma and tissues, and to compare the pharmacokinetics and tissue distribution of withanolide B in normal and psoriasis guinea pigs. After mixing with IS, plasma and tissues were pretreated by protein precipitation with methanol. Chromatographic separation was performed on a C18 column using aqueous (0.1% formic acid) and acetonitrile (0.1% formic acid) solutions at 0.4 mL/min as the mobile phase. The gradient program was selected. Detection was performed on a 4000 QTRAP UPLC–ESI-MS/MS system from AB Sciex in the multiple reaction monitoring (MRM) mode. Withanolide B and obakunone (IS) were monitored under positive ionization conditions. The optimized mass transition ion-pairs (m/z) for quantitation were 455.1/109.4 for withanolide B and 455.1/161.1 for obakunone.

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