Bessa-Gonçalves et al2021_Cytokine array data

Published: 29 December 2021| Version 1 | DOI: 10.17632/x4ychgbcv7.1
Contributor:
Susana Santos

Description

The hypothesis underlying this data was that modifying Fibrinogen (Fg) scaffolds with Magnesium (Mg) incorporation could modulate macrophage polarisation towards M2 anti-inflammatory phenotype, in vitro and in vivo. Cytokine array data are provided for: - Human cytokine array. Cytokines present in conditioned media from macrophages cultured, for 13 days, in Fg and FgMg (10 mM) scaffolds in the presence/absence of M1 stimulation (LPS+INFg) were quantified at Eve Technologies Corp. (Calgary, AB, Canada) using the Human Cytokine Array/Chemokine Array 48-Plex: soluble CD40 ligand (sCD40L), epidermal growth factor (EGF), Eotaxin/CCL11, fibroblast growth factor 2 (FGF-2), FMS-related tyrosine kinase 3 ligand (Flt-3 ligand), Fractalkine/CX3CL1, granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), growth-regulated on alpha protein (GRO-⍺/CXCL1), interferon alpha-2 (IFN⍺2), interferon gamma (IFN𝛄), interleukin (IL)-1⍺, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IL-17E/IL-25, IL-17F, IL-18, IL-22, IL-27, interferon induced protein 10 (IP-10/CXCL10), monocyte chemoattractant protein (MCP)-1/CCL2, MCP-3/CCL7, macrophage colony-stimulating factor (M-CSF), macrophage-derived chemokine (MDC/CCL22), monokine induced by interferon gamma (MIG/CXCL9), macrophage inflammatory protein -1 alpha (MIP-1⍺/CCL3), macrophage inflammatory protein -1 beta (MIP-1β/CCL4), platelet derived growth factor (PDGF)-AA, PDGF-AB/BB, regulated upon activation, normal T cell expressed, and secreted (RANTES/CCL5), transforming growth factor alpha (TGF⍺), tumor necrosis factor (TNF)⍺, TNFβ and vascular endothelial growth factor A (VEGF-A). The sensitivity of the assay for the analysed markers ranged from 0.14 to 50.78 pg/mL. - Mouse cytokine array. Cytokines present in inflammatory exudates were quantified at Eve Technologies Corp. (Calgary, AB, Canada) using the using the Mouse Cytokine Array/Chemokine Array 44-Plex: Eotaxin/CCL11, Erythropoietin, 6Ckine/CCL21, Fractalkine/CX3CL1, G-CSF, GM-CSF, interferon beta 1 (IFNB1), IFN𝛄, IL-1⍺, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-16, IL-17, IL-20, IP-10, keratinocyte chemoattractant (KC), leukemia inhibitor factor (LIF), lipopolysaccharide-induced CXC chemokine (LIX/CXCL5), MCP-1/CCL2, MCP-5/CCL12, M-CSF, MDC/CCL22, MIG/CXCL9, MIP-1⍺/CCL3, MIP-1β/CCL4, MIP-2/CXCL2, MIP-3⍺/CCL20, MIP-3B/CCL19, RANTES, thymus- and activation-regulated chemokine (TARC/CCL17), tissue inhibitor of metalloproteinase (TIMP)-1, TNFα, and VEGF. The sensitivity of the assay for the analysed markers ranged from 0.1 to 33.3 pg/mL. Both data for a pilot run (performed to determine if sample concentration was appropriate) and a run for all the other samples are provided. The sample identification legend is provided in all the files.

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Universidade do Porto Instituto de Investigacao e Inovacao em Saude

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Cytokines

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