Preparation of filamentous cyanobacteria for use with a droplet-based microfluidic device

Published: 6 June 2022| Version 1 | DOI: 10.17632/x6xfs9zm6k.1
Marwa Gamal Saad, Schonna Manning


This data set includes three videos of Oscillatoria acuminata collected from Port Said, Egypt. Cultures were prepared for microfluidic applications to observe its growth over time. Various filament lengths were generated (40 μm, 70 μm, and 100 μm) by sonication and captured inside 290 μm-droplets. Growth of filaments was observed for three days under different temperatures (20°C, 27°C, and 37 °C). During the observation period, the filaments were actively growing and entangled inside the droplets. The surface tension was high and contained the filaments inside the droplets without rupturing. In some cases, filaments fragmented inside the droplets to form hormogonia for continued growth.



Texas A&M University Central Texas, Port Said University, University of Texas at Austin


Biotechnology, Cell Biology, Microfluidics, Cyanobacteria, Algal Culture, Oil-Water Emulsion