MicroCT of stained bovine IVD quarter segments after 2 weeks incubation
Visualising the 3D microstructure of stained and native intervertebral discs using X-ray microtomography C.M. Disney 1,2, K. Madi 3, A.J. Bodey 4, P.D. Lee 3, J.A. Hoyland 2,5, M.J. Sherratt 2 1 Centre for Doctoral Training in Regenerative Medicine, 2 Division of Cell Matrix Biology and Regenerative Medicine, 3 School of Materials, University of Manchester, UK. 4 Diamond Light Source, Harwell Science and Innovation Campus, Oxfordshire, UK. 5 NIHR Manchester Biomedical Research Centre, Central Manchester University Hospitals NHS Foundation Trust, Manchester Academic Health Science Centre, UK. A comparison of three stains; iodine potassium iodide (I2KI), phosphomolybdic acid (PMA) and phosphotungstic acid (PTA). Chemically fixed (10% formal saline) quarter segments of bovine tail intervertebral disc were incubated for two weeks before being imaged by laboratory microCT. Reconstruction software (Phoenix dato s|x2 reconstruction) was used to generate 3D datasets from the projections. This reconstructed data relates to figure 3 and is saved as 8-bit .raw with image dimensions given in the file name.