MicroCT of stained bovine IVD quarter segments after 2 weeks incubation

Published: 12 Jun 2018 | Version 1 | DOI: 10.17632/x97x865jpm.1

Description of this data

Visualising the 3D microstructure of stained and native intervertebral discs using X-ray microtomography

C.M. Disney 1,2, K. Madi 3, A.J. Bodey 4, P.D. Lee 3, J.A. Hoyland 2,5, M.J. Sherratt 2

1 Centre for Doctoral Training in Regenerative Medicine, 2 Division of Cell Matrix Biology and Regenerative Medicine, 3 School of Materials, University of Manchester, UK.

4 Diamond Light Source, Harwell Science and Innovation Campus, Oxfordshire, UK.

5 NIHR Manchester Biomedical Research Centre, Central Manchester University Hospitals NHS Foundation Trust, Manchester Academic Health Science Centre, UK.

A comparison of three stains; iodine potassium iodide (I2KI), phosphomolybdic acid (PMA) and phosphotungstic acid (PTA). Chemically fixed (10% formal saline) quarter segments of bovine tail intervertebral disc were incubated for two weeks before being imaged by laboratory microCT. Reconstruction software (Phoenix dato s|x2 reconstruction) was used to generate 3D datasets from the projections. This reconstructed data relates to figure 3 and is saved as 8-bit .raw with image dimensions given in the file name.

Experiment data files

Latest version

  • Version 1


    Published: 2018-06-12

    DOI: 10.17632/x97x865jpm.1

    Cite this dataset

    Disney, Catherine (2018), “MicroCT of stained bovine IVD quarter segments after 2 weeks incubation”, Mendeley Data, v1 http://dx.doi.org/10.17632/x97x865jpm.1


Views: 73
Downloads: 12


The University of Manchester


3D Imaging, X-Ray Imaging


CC BY 4.0 Learn more

The files associated with this dataset are licensed under a Creative Commons Attribution 4.0 International licence.

What does this mean?
You can share, copy and modify this dataset so long as you give appropriate credit, provide a link to the CC BY license, and indicate if changes were made, but you may not do so in a way that suggests the rights holder has endorsed you or your use of the dataset. Note that further permission may be required for any content within the dataset that is identified as belonging to a third party.