The SMC-family Wadjet complex protects bacteria from plasmid transformation by recognition and cleavage of closed-circular DNA

Published: 22 August 2022| Version 1 | DOI: 10.17632/x9923grfjw.1
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Description

Bacterial Wadjet defense system protect the host cell from transformation by plasmid DNA. In the related manuscript of this dataset, we identified that Wadjet complex specifically recognizes and cleaves the circular DNA in a reaction requiring ATP. Using CryoEM, cross-linking mass spectrometry and biochemical assays, here we resolved the overall architecture of full Wadjet complex.

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The experimental details are reported in the method section of linked paper.

Institutions

University of California San Diego

Categories

Biochemistry, Molecular Biology, Structural Biology

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