Rational Design of Evolutionarily Stable Microbial Kill Switches. Finn Stirling et al.

Published: 10-08-2017| Version 1 | DOI: 10.17632/xgbjnmxnbr.1
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Finn Stirling

Description

GFP expression in a temperature sensitive fashion in E. coli. figure 4 of Rational Design of Evolutionarily Stable Microbial Kill Switches. Finn Stirling et al.

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Steps to reproduce

express GFP under the temperature sensitive promoter PcspA. place on plasmid vector pUA66. grow at 37, 30, 22 and 15 degrees centigrade on LB plates. use PrpsL GFP, theta GFP and no plasmid as controls.