Grentzinger_TraPR_RAW_Data

Published: 11 Sep 2019 | Version 1 | DOI: 10.17632/xgfhrtbw48.1
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Description of this data

A universal method for the rapid isolation of all known classes of functional small RNAs.

Diverse classes of regulatory small (s)RNAs operate via ARGONAUTE-family proteins within RNA-induced-silencing-complexes (RISCs). Based on the conserved biochemical properties intrinsic to all ARGONAUTEs, we have developed a universal, 15-min benchtop extraction procedure allowing simultaneous purification of all classes of RISC-associated sRNAs, without prior knowledge of the sample’s -intrinsic ARGONAUTE repertoires. Optimized as a user-friendly kit, the method –coined “TraPR” for Trans-kingdom, rapid, affordable Purification of RISCs– operates irrespectively of the organism, tissue, cell type or bio-fluid of interest, and scales to minute amounts of input material. The method is highly suited for direct sRNA profiling, with TraPR-generated libraries outperforming those obtained via gold-standard procedures that require immunoprecipitations and/or lengthy polyacrylamide gel excisions. TraPR considerably improves the quality and consistency of sRNA sample preparation including from notoriously difficult-to-handle tissues/bio-fluids such as starchy storage roots and mammalian plasma, and regardless of RNA contaminants or samples’ RNA-degradation status.

Experiment data files

Latest version

  • Version 1

    2019-09-11

    Published: 2019-09-11

    DOI: 10.17632/xgfhrtbw48.1

    Cite this dataset

    GRENTZINGER, Thomas (2019), “Grentzinger_TraPR_RAW_Data”, Mendeley Data, v1 http://dx.doi.org/10.17632/xgfhrtbw48.1

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Categories

Molecular Biology, MicroRNA, RNA, RNA Interference

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The files associated with this dataset are licensed under a Creative Commons Attribution 4.0 International licence.

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