Impact of Norway spruce pre-degradation stages induced by G. trabeum on fungal and bacterial communities

Published: 18 July 2022| Version 3 | DOI: 10.17632/xhm5nrn8cr.3
Contributor:
Nicolas Valette

Description

The supplementary data for the article titled "Impact of Norway spruce pre-degradation stages induced by G. trabeum on fungal and bacterial communities". Supporting information file containing figure S1 (most abundant fungal OTUs (127) displayed at least 1 % abundance in at least one sample. The fungal OTUs were described at the species level and 11 of them were chosen (yes in red) to see a putative co-occurrence pattern with bacteria), figure S2 (Most abundant bacterial OTUs (116) displayed at least 1 % abundance in at least one sample. The bacterial OTUs were described at the family level and 30 of them were chosen (yes in red) to see a putative co-occurrence pattern with fungi), figure S3 (the abundance relative of Gloeophyllum trabeum found in each environment after data sequencing analysis), figure S4 ((A) Plot planted with Norway spruce selected for the study. (B) Experimental design with the location of the wood blocks in relation to the forest floor litter layer, and (C) Soil core sampling from selected area (90 x 45 cm) used for the study), and figure S5 (the photo illustrates the place where the samples were buried (red square representing the area of 90 x 45 cm) during 70 days at the Breuil-Chenue forest). Table S1. (A) Wood blocks degraded by Gloeophyllum trabeum, (B) chemical composition according to mass loss of wood blocks and (C) wood blocks incubated in situ. Table S2. PERMANOVA analysis to measure the impact of environment types, the laboratory pre-degradation and the different pre-degradation stages on microbial community structures. Table S3. Statistical analysis on the fungal and bacterial taxonomic abundance: (A) PERMANOVA analysis on microbial communities at different taxonomic level, (B) Pairwise comparison (T-test) of bacterial family abundance (p-value adjust method (holm)) between pre-degradation, non-decayed wood, soil and forest, (C) Pairwise comparison (T-test) of fungal genus abundance (p-value adjust method (holm)) between pre-degradation, non-decayed wood, soil and forest, (D) Pairwise comparison (T-test) of bacterial family abundance (p-value adjust method (holm)) between intermediate and advanced pre-degraded wood, and (E) Pairwise comparison (T-test) of fungal genus abundance (p-value adjust method (holm)) between non-, intermediate and advanced pre-degraded wood. Table S4. (A) Spearman rank correlation matrix between the abundance of 30 bacterial OTUs and 11 fungal OTUs, and (B) the associated p-value. Table S5. Site characteristics: (A) soil properties, (B) forest floor litter layerchemical properties and (C) C/N ratio of non-decayed wood. Table S6. (A) Bacterial and (B) Fungal OTU tables.

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Molecular Method for Microbial Ecology Studies

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