Baghdadi_Cell Stem Cell_D-17-00836_2018
Description
Supplementary Movie 1: Related to Fig. 3C and D Cell trajectories of a 48h time-lapse movie of membrane-GFP positive cells isolated from resting muscle of Tg:Pax7-CT2;R26mTmG mice transfected immediately after FACS with Scramble control (top) or Mimic-708 (bottom). Images were taken every 13min. Scale bar: 50μm. Supplementary Movie 2 and 3: Related to Fig. 3E and F 2-Photon live videomicroscopy on Extensor digitorium longus muscle of Scramble-treated mice (10days post-injection). Representative zoom on cells showing no cell displacement over 12h. Images were taken every 30min. Supplementary Movie 4 and 5: Related to Fig. 3E and F 2-Photon live videomicroscopy on Extensor digitorium longus muscle of AntagomiR-treated mice (10days post injection). Representative zoom on cells showing spontaneous displacement and exit from niche following miR-708 knock-down in vivo. Images were taken every 30min. Supplementary Movie 6: Related to Fig. 3J Cell trajectory of primary satellite cells overexpressing miR-708 (Mimic) only or both miR-708 and TNS3. Overexpression of TNS3 was sufficient to rescue the inhibition of migration mitigated by mir-708. Images were taken every 10min for 20h. Supplementary Movie 7: Related to Fig. 4F-H Videomicroscopy of a Fucci-green-positive cell isolated from resting muscle and transfected 24h after FACS with Scramble control (top) or Mimic-708 (bottom). Images were taken every 13min for 25h.