Raw data from: Effectiveness of the production of tissue-engineered living bone graft – a comparative study using perfusion and rotating bioreactor systems

Published: 21 April 2023| Version 1 | DOI: 10.17632/xj8mpsdt6k.1
Contributors:
Paulina Kazimierczak,
Grzegorz Kalisz,
Anna Sroka-Bartnicka,
Agata Przekora

Description

The primary goal of the research was to compare the effectiveness of 2 bioreactors: (1) perfusion (Lazar Arrow-MTM Micro Bioreactor System) and rotating (Rotary Cell Culture System) in the generation of a living bone construct to select the system that is more favorable for cell cultivation and their differentiation towards mature bone cells. For this purpose bone marrow-derived mesenchymal stem cells (BMDSCs) were seeded on the surfaces of hydroxyapatite-based scaffolds and cultured in three different conditions: (1) static 3D culture – a test control (mat_S), (2) 3D culture in a perfusion bioreactor (mat_P), and (3) dynamic 3D culture in a rotating bioreactor (mat_R). Cell growth on the scaffolds was assessed quantitatively by LDH total assay and qualitatively by live/dead staining, cytoskeleton staining, and SEM imaging. Osteogenic differentiation was compared by ELISAs and immunofluorescent staining, whereas ECM mineralization was determined using Raman spectroscopy. Folders: ‘cytoskeleton_staining 1d’ and ‘cytoskeleton_staining 21d’ contain raw CLSM images after cytoskeleton staining after 1 day (before placing the samples into the bioreactors) and 21 days of culture, respectively. Folder ‘live dead” contains raw CLSM images of the cells on the scaffold after live/dead staining (before placing the samples into the bioreactors). Folder ‘IF osteonectin_collagen” contains CLSM images after immunofluorescent staining of osteogenic markers. Folder ‘SEM’ contains raw SEM images after 21 days of culture. The name of the CLSM or SEM images indicates the specific sample and culture conditions, i.e. mat_S means static 3D culture, (2) mat_P means 3D culture in a perfusion bioreactor, and (3) mat_R means dynamic 3D culture in a rotating bioreactor. Folder ‘Raman_maps’ contains various Microsoft Excel (.csv) files. Each Microsoft Excel file contains the raw data for Raman mapping. Folder ‘Raman_spectra’ contains various Microsoft Excel (.csv) files. Each Microsoft Excel file contains the raw data for Raman spectra. For Raman data, file name indicates the type of the sample, i.e. mat_S, mat_P, mat_R, mat_control that means non-seeded biomaterial incubated in the culture medium, and native that means untreated biomaterial. Folder ‘ELISA_osteogenic markers’ contains various Microsoft Excel (.xlsx) files. Each Microsoft Excel file contains the raw results of ELISA for osteogenic marker (file name indicates the type of osteogenic marker). ELISA results are shown as concentration of specific osteogenic marker. Column B shows the name of the sample (mat_S, mat_P or mat_R). The Microsoft Excel (.xlsx) file named ‘LDH_proliferation’ contains the raw results of LDH total proliferation assay performed after 1 day of culture (before placing the samples into the bioreactors) and after 21 days of culture in specific conditions.

Files

Institutions

Uniwersytet Medyczny w Lublinie

Categories

Biomaterials, Stem Cell, Regenerative Medicine, Bioreactor System, Scaffold for Tissue Engineering, Bone Tissue Engineering

Funding

Narodowe Centrum Nauki

UMO-2021/43/B/NZ7/00447

Ministerstwo Edukacji i Nauki

PBmb2/2021

License