Functional homology of tachykinin signalling: The influence of human substance P on the immune system of the mealworm beetle, Tenebrio molitor L. - comet assay raw dataset
Description
Raw datasets related to the DNA integrity assay published in article “Functional homology of tachykinin signalling: The influence of human substance P on the immune system of the mealworm beetle, Tenebrio molitor L.” (https://doi.org/10.1016/j.dci.2023.104669).
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The DNA integrity of T. molitor haemocytes was measured based on the method described by Lubawy et al. (2019). For this purpose, 7-days-old adult males were injected with human substance P (RPKPQQFFGLMa) solution in physiological saline at a concentration of 10-7 and 10-5 M (10-8 and 10-6 M in insect body, respectively). Control individuals were injected with physiological saline. Haemocytes of treated beetles were collected 6 or 24 hours after injection. Haemocytes treated with H2O2 served as a positive control for each step in the comet assay.. The raw data were generated by OpenComet software based on the method described by Gyori et al. (2014). In the manuscript only a basic parameters were shown and analysed. The dataset can be used for in-depth analysis of influance of human substance P on DNA integrity of insect cells. References; Gyori, B.M., Venkatachalam, G., Thiagarajan, P., Hsu, D., Clement, M.-V., 2014. OpenComet: an automated tool for comet assay image analysis. Redox Biol. 2, 457–465 Lubawy, J., Daburon, V., Chowański, S., Słocińska, M., Colinet, H., 2019. Thermal stress causes DNA damage and mortality in a tropical insect. J. Exp. Biol. 222
Institutions
- Uniwersytet im Adama Mickiewicza w Poznaniu
- Freie Universitat Berlin
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Funders
- Initiative of Excellence – Research UniversityGrant ID: 018/07/POB2/ 0001
- Initiative of Excellence – Research UniversityGrant ID: 011/08/POB2/0009