Microfluidic platform based on site-specific post-imprinting modification of molecularly imprinted monolith to improve identification of N-myristoylated peptides
In order to improve the identification of N-myristoylated peptides by mass spectrometry, an enrichment strategy based on microfluidic platform was presented by combining molecularly imprinted polymer (MIPs) with post-imprinting modification and trypsin-immobilized enzyme reactor (IMER). The resulting imprinted monolith was used as an apparatus for peptides enrichment in a microfluidic platform composed of a chip and IMER for treatment of the proteins extracted from MCF-7 cells. A total of 1,296 proteins and 5,670 peptides can be identified, including 71 myristoylated proteins and 78 myristoylated peptides. The uploaded compressed package included Origin data, Total myristoylated peptides, Total myristoylated proteins, Total peptides and proteins before fractionation, Total peptides and proteins in the effluent, rinse and eluent after fractionation.