HPLC-MS Data of Madder and Cochineal Pigments

Published: 20 November 2020| Version 1 | DOI: 10.17632/xnvf7gb66r.1
Jing Han, Beatriz Fonseca


Normalized peak area distributions of high performance liquid chromatography - mass spectrometry data of the madder and cochineal pigments made of different dye sources and with various recipes.


Steps to reproduce

Reference pigment samples of 0.5 mg were weighed for extraction by 4% boron trifluoride in methanol. An Agilent Technologies 1200 Series HPLC system coupled to a 1200 Photo Diode Array detector and a Bruker HCT (high capacity spherical traps) electronic spray ionization MS was used to analyze the extract from the samples. An Agilent Poroshell 120 EC C18 column (2.1×100 mm, particle size 2.7 µm) with a pre-column of the same type was used. The mobile phase comprised 0.1% formic acid in 95% aqueous methanol (v/v) (eluent A), and 0.1% formic acid in pure methanol (eluent B). Flow rate was 0.3 mL min-1 and column temperature was 40 °C. MS ionization was carried out in negative mode. Ultra Scan was used, with m/z range 50-1000 and speed 26,000 m/z second-1. Target Mass was set at 400 m/z. Data processing was performed using Bruker Daltonics DataAnalysis.


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