A Study of The nuclear export signal is required for cGAS to sense cytosolic DNA. Sun et al.
Published: 12 October 2020| Version 1 | DOI: 10.17632/xpjp4n4yjs.1
cGAS is located both in the cytoplasm and in the nucleus. cGAS nuclear accumulation was observed when leptomycin B was used to block the exportin, chromatin maintenance region 1 protein. Identified a functional nuclear export signal (NES) in cGAS, 169-LEKLKL-174. Mutating this NES led to sequestration of cGAS within the nucleus and the loss of interferon response to cytosolic DNA treatment.
Confocal Microscopy, Western Blot