A Study of The nuclear export signal is required for cGAS to sense cytosolic DNA. Sun et al.
cGAS is located both in the cytoplasm and in the nucleus. cGAS nuclear accumulation was observed when leptomycin B was used to block the exportin, chromatin maintenance region 1 protein. Identified a functional nuclear export signal (NES) in cGAS, 169-LEKLKL-174. Mutating this NES led to sequestration of cGAS within the nucleus and the loss of interferon response to cytosolic DNA treatment.