Comparison of oxygen induced retinopathy transcriptomes between Sprague Dawley rats from two vendors

Published: 22 December 2022| Version 1 | DOI: 10.17632/xrjrnjmydt.1
Contributor:
Ellen Ingolfsland

Description

To investigate variable susceptibilities to retinopathy of prematurity among infants, we performed RNA sequencing on whole retinal lysates from Sprague Dawley rat pups from Charles River Laboratories and Envigo that underwent the Penn 50/10 oxygen-induced retinopathy (OIR) model. Normoxia control and 50/10 OIR retinas underwent next-generation RNA sequencing, n=4/ group. Pairwise comparisons were performed to generate differentially expressed genes between OIR and control groups from the two vendors during the avascular phase of OIR (postnatal day 15) and the neovascular phase (postnatal day 20).

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Institutions

University of Minnesota

Categories

Retina, Retinopathy of Prematurity, Next Generation Sequencing

Funding

American Academy of Pediatrics

Knights Templar Eye Foundation

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