Primary Uncropped Images and Biological Replicates for DOI: 10.1016/j.nbd.2018.09.006
Description
This dataset contains raw source files, biological replication cohorts, and funding verifications for DOI: 10.1016/j.nbd.2018.09.006. The evidence proves published figures and historical grant applications represent independent experiments run on separate timelines. The repository is structured into four operational subfolders: 1. Original_Uncropped_Blots_Manuscript: Uncropped primary TIFF source files for finalized cohorts (e.g., 'CLDN5 young old bv.tif', 'GAPDH young old_bv.tif'). These verify distinct lane configurations and background architecture. 2. Biological_Replicates_2017_Repeats: Baseline replication datasets validating final figures (n=5 for Western blots; n=3 for co-immunoprecipitation), serving as structural proof of independent sample cohorts. 3. Historical_Grant_Source_Files: Early-stage, single-replicate (n=1) pilot runs generated years prior solely for exploratory funding applications (e.g., 'CO_occludin_y_o_SAMPy_o_SAMP 30 sec.tif', 'Co ZO1_cldn5_y_o_SAmp_1.tiff'). 4. Grant_Verification_and_Timeline_Documentation: Official administrative extracts validating timelines. Includes '4016280_Egrant.pdf' (RF1AG057928), 'EgrantAAZ.pdf' (1R01NS099161), '1-16-IBS-008_Andjelkovic-Zochowska.pdf', and 'ADADKAF.docx' (recovered backup documenting a 2018 local storage drive failure). These materials are placed in the public domain to maintain transparency and address a critical procedural omission in the institutional review process, which declined to audit or evaluate this raw primary evidence. This public dataset contains the data tracking framework, core uncropped source files, and funding verifications for DOI: 10.1016/j.nbd.2018.09.006. The physical evidence proves that the published figures and historical grant applications represent entirely independent biological experiments run on separate timelines. STORAGE CAP ACTIY NOTE: Due to platform capacity restrictions, the complete high-resolution multi-replicate instrument files for this study exceed 10GB. The primary tracking metadata, grant files, and core text documents are hosted natively here.
Files
Steps to reproduce
1. ACCESS THE 10GB DATA HOUSING: Click the external open-access cloud link provided in the description [PASTE YOUR HEAVY CLOUD LINK HERE] to download the complete raw data package consisting of three distinct subfolders. 2. AUDIT FOLDER 1 (Original_Images_Data): Cross-reference the cropped figure panels in the published manuscript with the full-length, uncropped primary instrument captures ('CLDN5 young old bv.tif' and 'GAPDH young old_bv.tif') to verify multi-replicate biological sample cohorts (n=5 for Western blots; n=3 for co-immunoprecipitation). 3. AUDIT FOLDER 2 (Grants_Part_1): Examine the historical exploratory pilot files ('CO_occludin_y_o_SAMPy_o_SAMP 30 sec.tif' and 'Co ZO1_cldn5_y_o_SAmp_1.tiff') to verify that the early grant applications relied strictly on early-stage, single-replicate (n=1) pilot runs from a separate animal cohort. 4. AUDIT FOLDER 3 (Grants_Part_2): Review the official NIH Egrant files ('4016280_Egrant.pdf' for RF1AG057928 and 'EgrantAAZ.pdf' for 1R01NS099161) alongside the recovered local text backup ('ADADKAF.docx') to cross-reference chronological separation and verify the 2018 laboratory hardware drive failure data recovery path.