Data for: Continued expansion of tick-borne pathogens: Tick-borne encephalitis virus complex and Anaplasma phagocytophilum in Denmark
This dataset comprises information on Danish roe deer (Capreolus capreolus). All eligible samples were examined for the presence of Tick-borne encephalitis virus (TBEV) neutralizing antibodies. A selected group of roe deer was also tested for the infectious rate of Anaplasma phagocytophilum. Danish hunters were asked to obtain blood samples from the thoracic cavity of roe deer when dressing freshly killed animals in the field, during the hunting season of 2013 and 2014 (October 1, 2013-January 31, 2014 [Autumn/Winter] and May 15–July 15, 2014 [Spring/Summer]). A small portion of the roe deer was killed as part of a tracking hound operation for wounded or traffic injured game and was therefore sampled between the two hunting seasons.
Steps to reproduce
Tick-borne encephalitis virus neutralization test All samples were examined for TBEV-specific antibodies by TBEV neutralization tests (NT) at the Department of Virology, Medical University Vienna, Austria as described by Stiasny, K., Holzmann, H., Heinz, F.X., 2009. Characteristics of antibody responses in tick-borne encephalitis vaccination breakthroughs. Vaccine 27, 7021–7026. https://doi.org/10.1016/j.vaccine.2009.09.069 <i>Anaplasma phagocythophilum</i> PCR A selected group of samples was examined for the presens of <i>Anaplasma phagocytophilum</i> by Real-Time PCR at the Department of Clinical Microbiology, Odense University Hospital, Odense, Denmark modiefied to our laboratory conditions after Courtney, J.W., Kostelnik, L.M., Zeidner, N.S., Massung, R.F., 2004. Multiplex Real-Time PCR for Detection of Anaplasma phagocytophilum and Borrelia burgdorferi. J. Clin. Microbiol. 42, 3164–3168. https://doi.org/10.1128/JCM.42.7.3164-3168.2004